The U.S. Environmental Protection Agency added whole effluent toxicity testing to the Discharge Monitoring Report/Quality Assurance (DMRQA) Study in 1991. Aquatox Research personnel have participated in the toxicity testing portion of the annual program since it's inclusion both prior to and following our group's divestiture from Syracuse Research Corporation. Laboratories generating data in support of NPDES and SPDES permits are required to participate in the study. Laboratories are only required to conduct the tests for which data are generated during the year. Data provided below only reflect results generated in the Aquatox Research facility relating to routine freshwater effluent tests and organisms used in New York State. Gaps in chronic test results reflect years in which no clients requested those tests in permit-related testing.

DMRQA STUDY RESULTS FOR AQUATOX RESEARCH, INC.
Fathead minnow, Pimephales promelas, Acute (48-hr) Test Results
Conducted in Moderately-Hard Reconstituted Water at 20°C.
STUDY
NO.
YEAR
TEST
ENDPOINT
ASSIGNED
VALUE
(%)
ACCEPTANCE
LIMITS
(%)

Aquatox Research REPORTED
VALUE
(%)

DECISION
033 2013 LC50 17.0 7.46 - 26.6 18.9 Acceptable
032 2012 LC50 21.0 5.95 - 36.0 31.9 Acceptable
031 2011 LC50 19.2 7.08 - 31.4 30.8 Acceptable
030 2010 LC50 27.9 8.17 - 47.6 36.9 Acceptable
029 2009 LC50 31.3 11.4 - 50.1 **51.8**a Not Acceptable
028 2008 LC50 30.4 0.00 - 65.3 33.0 Acceptable
027 2007 LC50 - 0.00 - 60.1 36.9 Acceptable
026 2006 LC50 48.6 <6.25->100 70.7 Acceptable
025 2005 LC50 20.8 <6.25->53.8 12.5 Acceptable
024 2004 LC50 59.3 <6.25->100 11.1 Acceptable
023 2003 LC50 31.7 14.1-49.3 31.0 Acceptable
a Exceedance attributed to above average size fish (=more tolerant) used in testing despite the appropriate age. Adopted policy of using fish from midrange (6-8 days old) of accepted age range (1-14 days old) for DMRQA tests.
DMRQA STUDY RESULTS FOR AQUATOX RESEARCH, INC.
Fathead minnow, Pimephales promelas, Chronic (7-day) Test Results
Conducted in Moderately-Hard Reconstituted Water at 25°C.
STUDY
NO.
YEAR
TEST
ENDPOINT
ASSIGNED
VALUE
(%)
ACCEPTANCE
LIMITS
(%)

Aquatox Research REPORTED
VALUE
(%)

DECISION
033 2013 NOEC/SRV 12.5 6.25-25 25.0 Acceptable
NOEC/GR 12.5 6.25-25 12.5 Acceptable
IC25/GRW 23.4 7.34-39.5 14.7 Acceptable
032 2012 NOEC/SRV 25.0 12.5-50.0 25.0 Acceptable
NOEC/GR 25.0 12.5-50.0 25.0 Acceptable
IC25/GRW 29.4 9.33-49.4 17.1 Acceptable
031 2011 NOEC/SRV 25.0 12.5-50.0 12.5 Acceptable
NOEC/GR 25.0 12.5-50.0 12.5 Acceptable
IC25/GRW 27.2 11.9-42.4 29.3 Acceptable
030 2010 NOEC/SRV 25.0 12.5-50.0 25.0 Acceptable
NOEC/GR 25.0 6.25-50.0 25.0 Acceptable
IC25/GRW 26.6 <6.25-49.6 25.9 Acceptable
029 2009 NOEC/SRV 25.0 12.5-50.0 25.0 Acceptable
NOEC/GR 12.5 6.25-50.0 12.5 Acceptable
IC25/GRW 23.0 7.16-38.9 26.5 Acceptable
028 2008 NOEC/SRV 25.0 12.5-50.0 50.0 Acceptable
NOEC/GR 25.0 12.5-50.0 12.5 Acceptable
IC25/GRW 33.7 <6.25-62.1 20.4 Acceptable
027 2007 NOEC/SRV - 12.5-50.0 25.0 Acceptable
NOEC/GR - 12.5-50.0 12.5 Acceptable
IC25/GRW - 3.68-74.1 33.7 Acceptable
026 2006 NOEC/SRV 25.0 12.5-50.0 25.0 Acceptable
NOEC/GRW 25.0 12.5-50.0 25.0 Acceptable
IC25/GR 38.3 0.00-87.9 30.2 Acceptable
025 2005 NOEC/SRV 25.0 12.5-50.0 25.0 Acceptable
NOEC/GRW 25.0 12.5-50.0 12.5 Acceptable
IC25/GRW 32.9 6.90-58.9 32.9 Acceptable
024 2004 NOEC/SRV 25.0 12.5-50.0 12.5 Acceptable
NOEC/GRW 25.0 12.5-100 12.5 Accept
IC25/GRW 39.8 <6.25-76.4 19.6 Acceptable

DMRQA STUDY RESULTS FOR AQUATOX RESEARCH, INC.
Ceriodaphnids, Ceriodaphnia dubia, Acute (48-hr) Test Results
Conducted in Moderately-Hard Reconstituted Water at 25°C.
STUDY
NO.
YEAR
TEST
ENDPOINT
ASSIGNED
VALUE
(%)
ACCEPTANCE
LIMITS
(%)

Aquatox Research REPORTED
VALUE
(%)

DECISION
033 2013 LC50 61.1 18.4-104 61.6 Acceptable
032 2012 LC50 46.1 7.23-85.1 33.0 Acceptable
031 2011 LC50 27.5 3.06-52.0 19.1 Acceptable
030 2010 LC50 30.6 <6.25-58.0 17.7 Acceptable
029 2009 LC50 32.0 4.97-59.1 28.7 Acceptable
028 2008 LC50 36.0 0.00-83 13.5 Acceptable
027 2007 LC50 - 0.00->100 50.0 Acceptable
026 2006 LC50 32.2 <6.25-68.2 18.2 Acceptable
025 2005 LC50 32.2 <6.25-68.2 18.2 Acceptable
024 2004 LC50 37.5 <6.25-70.3 29.7 Acceptable
023 2003 LC50 50.2 4.37-96.1 39.2 Acceptable

DMRQA STUDY RESULTS FOR AQUATOX RESEARCH, INC.
Ceriodaphnids, Ceriodaphnia dubia, Chronic (7-day) Test Results
Conducted in Moderately-Hard Reconstituted Water at 25°C.
STUDY
NO.
YEAR
TEST
ENDPOINT
ASSIGNED
VALUE
(%)
ACCEPTANCE
LIMITS
(%)

Aquatox Research REPORTED
VALUE
(%)

DECISION
033 2013 NOEC/SRV 12.5 6.25-25 12.5 Acceptable
NOEC/REP 6.25 <6.25-12.5 <6.25 Acceptable
IC25/REP 9.90 0.854-19.8 4.17 Acceptable
032 2012 NOEC/SRV 25.0 12.5-50.0 25.0 Acceptable
NOEC/REP 12.5 6.25-25.0 12.5 Acceptable
IC25/REP 23.1 2.27-43.9 16.1 Acceptable
031 2011 NOEC/SRV 25.0 12.5-50.0 25.0 Acceptable
NOEC/REP 12.5 6.25-25.0 12.5 Acceptable
IC25/REP 18.0 5.15-30.8 15.0 Acceptable
030 2010 NOEC/SRV 25.0 12.5-50.0 25.0 Acceptable
NOEC/REP 25.0 <6.25-25.0 12.5 Acceptable
IC25/REP 20.3 <6.25-39.3 22.0 Acceptable
029 2009 NOEC/SRV 25.0 <6.25-49.6 25.0 Acceptable
NOEC/REP 12.5 6.25-25.0 12.5 Acceptable
IC25/REP 12.5 0.00-26.0 10.3 Acceptable
028 2008 NOEC/SRV 50.0 25.0-100 50.0 Acceptable
NOEC/REP 25.0 12.5-50.0 25.0 Acceptable
IC25/REP 31.9 7.70-56.1 24.6 Acceptable
027 2007 NOEC/SRV Did Not Conduct
NOEC/GR
IC25/GRW
026 2006 NOEC/SRV 25.0 00.0-50.0 25.0 Acceptable
NOEC/SRV 25.0 6.25-50.0 25.0 Acceptable
IC25/REP 27.5 7.99-46.9 21.3 Acceptable
025 2005 NOEC/SRV 25.0 12.5-50.0 50.0 Acceptable
NOEC/REP 25.0 6.25-50.0 25.0 Acceptable
IC25/REP 26.4 <6.25-53.6 28.8 Acceptable
024 2004 NOEC/SRV 25.0 12.5-50.0 50.0 Acceptable
NOEC/REP 25.0 6.25-50.0 25.0 Acceptable
IC25/REP 25.9 <6.25-53.5 25.5 Acceptable